Intracellular cytosolic Ca 2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca 2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca 2+ . On the other hand, by using Luminocounter with apoaequorin system, a method based upon luminescent photoprotein aequorin, intracellular Ca 2+ concentration was accumulated as a consequence of calcium

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... phenotype of S. cerevisiae. This fact indicated that PPase ptp2Δ Δ Δ Δ Δ and msg5Δ Δ Δ Δ Δ were involved in intracellular Ca 2+ transport in addition their already known pathways i.e Mitogen Activated Protein Kinase cell wall integrity pathway, high osmolarity glycerol (HOG) pathway, and pheromone response FUS3 pathway.