Mechanical modulation of a human plasma based skin scaffold via reactive multi-arm polyethylene glycols

2018 Biomecánica  
Autologous human plasma-based dermo-epidermal skin equivalents have been designed over the past decade to treat burns and surgical wounds; however, poor mechanical properties including fragility during handling, and shrinking during culture and implantation persist and demand creative solutions. This article investigates the mechanical modulation of human plasma-based skin scaffolds with the introduction of an interpenetrating biodegradable polyethylene glycol (PEG) network composed of 4-arm
more » ... cinimidyl glutarate terminated PEG (4SG-PEG) and 4-arm amine terminated PEG (4A-PEG), each with a molecular weight of 10 kDa. The physical properties of the PEG-fibrin network hydrogels were characterized via gelation time, temporal mass swelling, and protein release. Additionally, viability of primary human fibroblasts (hFBs) and keratinocytes (hKCs) was assessed by Alamar Blue and MTS assays, respectively. The results obtained suggested that the incorporation of reactive multi-arm PEGs as an interpenetrating biodegradable PEG network modulated the physical behaviour by inducing swelling and increasing scaffold strength without significantly decreasing cell viability. These results warrant further investigation into this platform as an approach to influence skin scaffold properties.
doi:10.5821/sibb.24.1.5142 fatcat:h3xs4gfwhba5xazoot5nkm2lfm