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An antibody that recognizes tyrosine hydroxylase can be used as a marker for hatching gland cells in Xenopus embryos. Using this marker, we have shown that hatching gland cells are induced at the end of gastrulation and that presumptive hatching gland cells are localized to the anterior neural folds in Xenopus. The movements of neurulation bring the hatching gland cells together to form a characteristic Y pattern on the dorsoanterior surface of the head. The Y pattern delineates several zonespmid:1680048 fatcat:gmgbwl2usrdw3lvpswsd5fg7ae