Comparative study of human papilloma virus DNA detection and results of histopathological examination of cervical colposcopic biopsy

Ahmad Ragab, Shalaby, Ahmad Mostafa, Shalaby, Saed Naguib
2007 Iranian Journal of Reproductive Medicine   unpublished
There is mounting evidence for HPV involvement in cervical cancer Human Papilloma Virus DNA is detected by hybridization techniques in 75-100% of patients with condylomas, precancerous cervical dysplasia, and invasive carcinoma. Objective: The aim of this study was investigating factors that may contribute to false-negative colposcopic biopsy results in positive high-risk HPV DNA results. Material and Methods: Patients positive for high-risk human papillomavirus (HPV) DNA with negative cervical
more » ... h negative cervical histopathologic findings were examined between January 2004 and August 2006. Results: Patients with atypical squamous cells of undetermined significance (ASC) in Papanicolaou smears, with positive HPV DNA results, but negative cervical histopathologic findings accounted for 4.5% of all ASC smears submitted for HPV DNA testing. We found 4% of the cases had focal HPV infection or mild dysplasia. When serial sectioning of the biopsy material were examined, we found that 29% had clinically significant lesions: HPV infection or cervical intraepithelial neoplasia CIN 1, 18%; CIN II/III, 8%; and dysplasia, not otherwise specified (which we can not categorize into any group), 3%. Of the remaining patients, follow-up revealed squamous abnormalities in 25%. About 5% of patients with positive HPV DNA results had a negative follow-up biopsy result. "False-negative" biopsies accounted for one third of cases. Conclusion: In almost one third of cases, clinically significant lesions were found when additional levels were examined.