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Procedures to improve nuclear transplantation efficiency in the rabbit were evaluated. We report the influence of recipient oocyte age on the different steps of nuclear transplantation. The effect of multiple pulses and the influence of manipulation medium and cytochalasin B in the post-fusion/activation medium on activation and development were studied. Recently ovulated oocytes were enucleated at a higher rate (60%) than aged oocytes (3%,p < 0.005); they also fused at a higher rate (85% vs.doi:10.1095/biolreprod43.5.877 pmid:2291920 fatcat:ajofs53jxzakxn7ephse5dosm4