Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis

Bérengère J. C. Luthringer, Uma M. R. Katha, Regine Willumeit
2014 Journal of materials science. Materials in medicine  
10 Previous observations (e.g., decreased bacterial adhesion) have shed the light on the auspicious possibility to use 11 phosphatidylethanolamine as biomimetic coating for metal implants. Additionally, it was experimentally shown 12 that phosphatidylethanolamine induces bone formation, however, up to now no study was performed to 13 understand this observation or to find an explanation. In an attempt to unveil how and why 14 phosphatidylethanolamine can improve cell metabolism and osteogenic
more » ... fferentiation, primary cells (human 15 umbilical cord perivascular cells) were cultured on native or phosphatidylethanolamine coated surfaces. Several 16 parameters were followed on gene (real time polymerase chain reaction) and protein (e.g., dot-blot and ELISA 17 tests) levels. It was determined that phosphatidylethanolamine potentiates cell metabolism, osteogenic 18 differentiation, and mineralisation early processes. By preventing biofilm formation while promoting new bone 19 formation, phosphatidylethanolamine could be easily implemented as implant bio-mimicking coating. 20 (132words) 21 22 23 24 25
doi:10.1007/s10856-014-5263-8 pmid:24980874 fatcat:mk6lq6pwsvbrzobgcnax6zkh4u