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Effect of cysteine supplementation on in vitro maturation of bovine oocyte
2011
African Journal of Biotechnology
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This study was aimed at determining the effect of cysteine supplement during in vitro maturation of bovine oocytes. Cumulus-oocyte complexes (COCs) from abattoir ovaries were matured in vitro in Hepes-TCM 199 supplemented with 0.2 mM sodium pyruvate, 1 µg/ml 17-β-estradiol, 10% fetal calf serum (FCS), 0.5 µg/ml bFSH and 0 (control) and 100 or 500 µM/ml of cysteine for 24 h. When COCs matured in TCM 199 media with 500 µM/ml cysteine, the rate of maturation were increased as compared to the
doi:10.5897/ajb11.1686
fatcat:cwhmtsgftndjhhz7z2nl65d7gu