Evidence for transovarial transmission of tick-borne rickettsiae circulating in Northern Mongolia

Thomas C. Moore, Laura A. Pulscher, Luke Caddell, Michael E. von Fricken, Benjamin D. Anderson, Battsetseg Gonchigoo, Gregory C. Gray, Tao Lin
2018 PLoS Neglected Tropical Diseases  
Transstadial transmission of tick-borne rickettsiae has been well documented. Few studies, however, have evaluated the role of transovarial transmission of tick-borne rickettsiae, particularly in nature within the host-vector ecosystem. This cross-sectional study aimed to understand the role of transovarial transmission of tick-borne rickettsiae among feeding ticks at different life stages. Tick eggs laid by engorged wild-caught adult female ticks were pooled and tested for Rickettsia spp. and
more » ... naplasma/Ehrlichia spp. using molecular techniques, while adult fed ticks were tested individually. Additionally, larval and nymphal ticks were collected in the wild from small mammals, pooled and tested for Rickettsia spp. and Anaplasma/Ehrlichia spp. There were 38 fed adult and 618 larvae/nymphs (60 pools total) Dermacentor spp. ticks collected from livestock and rodents. All individual adult ticks and tick pools were positive for Rickettsia spp. While none of the larvae/nymphs were positive for Anaplasma/Ehrlichia spp., two adult fed ticks were positive. Rickettsia spp. DNA was detected in 91% (30/33) of the pooled eggs tested, and one pool of eggs tested positive for Anaplasma/Ehrlichia spp. Sequencing data revealed Rickettsia spp. shared !99% identity with R. raoultii ompA. Anaplasma/Ehrlichia spp. shared !89% identity with A. ovis 16S ribosomal RNA. This study identified potential transovarial transmission of Rickettsia spp. and Anaplasma spp. among D. nuttalli ticks. Additional studies are needed to further assess the proportion of transovarial transmission occurring in nature to better understand the burden and disease ecology of tick-borne rickettsiae in Mongolia. by the limited knowledge and understanding of tick and tick-borne rickettsiae ecology within Mongolia. Tick-borne rickettsiae of concern to humans and animals in this region of the world are Rickettsia spp., Anaplasma spp., and Ehrlichia spp. Using molecular techniques, we detected rickettsiae among all Dermacentor spp. tick life stages and demonstrated potential vertical transmission of Rickettsia spp., and Anaplasma spp. among wild engorged adult female Dermacentor nuttalli ticks. We believe our findings provide important information regarding the ecology of key rickettsiae associated with tick-borne disease in Mongolia.
doi:10.1371/journal.pntd.0006696 pmid:30148847 fatcat:eqyvkpbrgffntkou7kmx7pdzai