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Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the nodal meristems. The explants were sterilized using 0.1% HgCl 2 and cultured on Murashige and Skoog (MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP) was found suitable for bud breaking within four weeks. Kinetin (Kin) was notfatcat:42gq6lcmynfarcqxr6dvs4ozm4