A simple method for culture and stable maintenance of giant spheroplasts from the yeast Saccharomyces cerevisiae

Taihei Fukudomi, Tomohiro Kotani, Isamu Miyakawa
2011 Journal of General and Applied Microbiology  
When spheroplasts of the yeast Saccharomyces cerevisiae are cultured in liquid medium containing osmotic stabilizer, they undergo nuclear division and growth without cell division, resulting in the formation of giant spheroplasts with multinuclei. In this study, we report a simple method for the culture and stable maintenance of giant spheroplasts. The selection of culture media and cell concentration was found to be important for the growth and maintenance of giant spheroplasts. Among the
more » ... tions that we tested, static culture in a synthetic Burkholder's medium in 96-well U-bottomed culture plates was most effective. Under appropriate conditions, we could maintain giant spheroplasts for more than 6 days without proliferation of whole cells or marked lysis. The average diameter of spheroplasts can vary from 16 to 53 μm, depending on their initial concentration.
doi:10.2323/jgam.57.177 pmid:21914966 fatcat:2di6g7bnfzaf5bvfivgib6oske