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Mice derived from in vitro-αMEM cultured preimplantation embryos exhibit postprandial hyperglycemia and higher inflammatory gene expression in peripheral leukocytes

Shiori Ishiyama, Mayu Kimura, Nodoka Umihira, Sachi Matsumoto, Atsushi Takahashi, Takao Nakagawa, Teruhiko Wakayama, Satoshi Kishigami, Kazuki Mochizuki
2021 Bioscience, biotechnology and biochemistry  

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https://web.archive.org/web/20210216141332/https://watermark.silverchair.com/zbab023.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAArQwggKwBgkqhkiG9w0BBwagggKhMIICnQIBADCCApYGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM4PfN8-PXnJefVed7AgEQgIICZ_nQnpQfWl_ireYiTiyk2bPj4SdIXlsG4i3wfQQVVqXhfHxfVwkpLTjZl9hF0tPbsjFiKo4zbLPILCScMYVc6QTPOgcazXEag68HbcoN3mYMbR3LTMlbfWareH74Bd4rS92kH6aTkT4hhSWgmzoXk8snyv_PFAHbw3ifres4URpTbzrRf1MqbbA0vwdTr0HD__0iJG1qOJqLH_V_ORoZvKB4WIGVgm8djwTgN1BsC57FCnHhxiV0724qmGwzSWAzoQY6brDxi4OUEtg0uxUe-QvRit7rFfx7gSyD40S0LL0TNWM1ntFTDatcLoVKyi5igGHkqc-8yn95-i1YFGKGvUx4rSSLyi3nLvn_mXMdGIR1Juyj2LPf2tFIuVvuYxT09zmfYRgn7YfHnhBdEfdav0wYF8vM-VdXitGB3yaImjeaPhoM7qYX7xIGGXKCJUmh9nGi4t88kWoiMSKDcMW5f5-olwB0uD69IYZIMznlYXmIyw_pL12CtYgPDLeU62Jj-jb7VlzZGB_tCdu2o0l-oXQuKqXeZ-qfwHQtqrOIKfpegGhVWQmw4bvZvd-7wzHluhMs1WPA4t2wD0luIoSvyp3n8MlUEi_py83i0yDqJCmmTnNGaXk_-pdoMVNFCqeFOiNDPmSfks_YPevZ_YZRwb60X4v9KcPr3LaYzZDebK4W3y8uIdvhibAuB7vpIpmx7GlgS0-KBYY9uFxH73JREeCBHGdYr3jjw-9Y_aQDhXyOse0qElkmd4bU9-Hh3K5T2lPWYihhUYY585JKmUPAQrO9F3OAVX6w04FC34SYXXrbql9PwZPOAQ

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We examined whether peripheral leukocytes of mice derived from in vitro-αMEM cultured embryos and exhibiting type 2 diabetes had higher expression of inflammatory-related genes associated with the development of atherosclerosis. Also, we examined the impact of a barley diet on inflammatory gene expression. Adult mice were produced by embryo transfer, after culturing two-cell embryos for 48 h in either α-MEM (minimal essential media) or KSOM (potassium simplex optimized medium) control media.
more » ... e were fed either a barley or rice diet for 10 weeks. Postprandial blood glucose, and mRNA levels of several inflammatory genes, including Tnfa and Nox2, in blood leukocytes were significantly higher in MEM mice fed a rice diet compared with control mice. Barley intake reduced expression of S100a8 and Nox2. In summary, MEM mice exhibited postprandial hyperglycemia and peripheral leukocytes with higher expression of genes related to development of atherosclerosis, and barley intake reduced some gene expression.
doi:10.1093/bbb/zbab023 pmid:33587104 fatcat:wzr2nr76zzhdvjv3lzvdrekn6q
Citation

Shiori Ishiyama, Mayu Kimura, Nodoka Umihira, Sachi Matsumoto, Atsushi Takahashi, Takao Nakagawa, Teruhiko Wakayama, Satoshi Kishigami, Kazuki Mochizuki. "Mice derived from in vitro-αMEM cultured preimplantation embryos exhibit postprandial hyperglycemia and higher inflammatory gene expression in peripheral leukocytes." Bioscience, biotechnology and biochemistry (2021)