Correction: Analysis of FOXP3+Regulatory T Cells That Display Apparent Viral Antigen Specificity during Chronic Hepatitis C Virus Infection

Shuo Li, Stefan Floess, Alf Hamann, Silvana Gaudieri, Andrew Lucas, Margaret Hellard, Stuart Roberts, Geza Paukovic, Magdalena Plebanski, Bruce E. Loveland, Campbell Aitken, Simon Barry (+3 others)
2012 PLoS Pathogens  
We reported previously that a proportion of natural CD25 + cells isolated from the PBMC of HCV patients can further upregulate CD25 expression in response to HCV peptide stimulation in vitro, and proposed that virus-specific regulatory T cells (Treg) were primed and expanded during the disease. Here we describe epigenetic analysis of the FOXP3 locus in HCVresponsive natural CD25 + cells and show that these cells are not activated conventional T cells expressing FOXP3, but hardwired Treg with a
more » ... dwired Treg with a stable FOXP3 phenotype and function. Of ,46,000 genes analyzed in genome wide transcription profiling, about 1% were differentially expressed between HCV-responsive Treg, HCV-non-responsive natural CD25 + cells and conventional T cells. Expression profiles, including cell death, activation, proliferation and transcriptional regulation, suggest a survival advantage of HCV-responsive Treg over the other cell populations. Since no Treg-specific activation marker is known, we tested 97 NS3-derived peptides for their ability to elicit CD25 response (assuming it is a surrogate marker), accompanied by high resolution HLA typing of the patients. Some reactive peptides overlapped with previously described effector T cell epitopes. Our data offers new insights into HCV immune evasion and tolerance, and highlights the non-self specific nature of Treg during infection.
doi:10.1371/annotation/523d94fe-9730-499b-95b1-addd94089ff8 fatcat:s2zya7wcsnehxc4j5tk55wkqhy