Evaluation of two different laboratory methods for the identification of Aeromonas spp. in stool sample of patients with diarrhea

Mina Boustanshenas, Majid Akbari, Niloofar Rezaie
2016 Infection, Epidemiology and Medicine   unpublished
Aeromonas spp. can cause diarrhea and various infections in humans. Access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of Aeromonas species. The aim of this study was to evaluate two different methods including API 20E bacterial identification tests and the molecular detection using PCR primers specific for 16s-rRNA and 23S-rRNA genes sequences for identification of Aeromonas spp. in stool samples from patients with diarrhea. Materials
more » ... arrhea. Materials and Methods: One hundred stool samples from diarrheal patients were collected. All isolates were subjected toAPI 20 E strip tests and PCR using specific primers for identification of Aeromonas spp. Results: The API 20E analysis identified 2 (2.2%) isolates as Aeromonas spp. Molecular identification by aero-23S-rRNA gene confirmed the same 2 isolates as identified by the API 20E strips. Conclusion: Both API 20E system and PCR method using Aero 23S-rRNA primer were found to be accurate in identification of Aeromonas spp. isolates with highconfidence.
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