FRI0172 Inflammation on mri of the sacroiliac joints is highly predictive of structural damage in axial spondyloarthritis patients in clinical practice: data from the asas and desir cohorts

A. Sepriano, S. Ramiro, R. Landewé, M. Dougados, D. van der Heijde, M. Rudwaleit
2018 FRIDAY, 15 JUNE 2018   unpublished
evaluated by RT-PCR and real-time RT-PCR. Proliferative responses and cell cycle analysis were detected by a CCK-8 assay and flow cytometry, respectively. ALP activity and ALP mass were detected by an ALP activity assay and ALP staining, respectively. Results: In primary osteoblasts, only the IL-17 receptor was expressed. In C2C12, MC3T3-E1, and Saos-2 cells, the genes of IL-17, IL-22, and IL-23 receptors were not detectable. None of IL-17, IL-22, and IL-23 had an obvious effect on the
more » ... ect on the proliferation of primary osteoblasts, but IL-17 exhibited an inhibitory effect on the gene expression of ALP, OCN, and Runx-2. The ALP activity and ALP mass of primary osteoblasts were downregulated by IL-17 treatment in a dose-dependent manner. Conclusions: Primary osteoblasts constitutively express IL-17 receptors, but none of C2C12 cells, MC3T3-E1 cells, and Saos-2 cells express any receptors for IL-17, IL-22, and IL-23. IL-17 inhibits BMP-2-induced osteoblast differentiation.
doi:10.1136/annrheumdis-2018-eular.2419 fatcat:wtktnxqs3rh3xg7rsjhrnetwdi