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Optical Trapping and Optical Micromanipulation III
In this report, we combine total internal reflection-fluorescence correlation spectroscopy (TIR-FCS) with a single optical trap to simultaneously manipulate and measure the dynamics of individual molecules near the substrate-solution interface. As a proof of principle, polystyrene particles (84 nm in diameter) are used as a model system to test our approach in studying their diffusion properties near surfaces, which are treated with polyethylene glycol 8000, bovine serum albumin or sodiumdoi:10.1117/12.680771 fatcat:bqqbznzvhjbkjnfrefpe6y3sk4