Studies with conifer-infesting bark beetles commonly use bolts cut from trees to evaluate the effects of host tree quality on various aspects of insect biology. Yet, whether host quality changes between live trees and bolts cut from these trees has not been assessed. Particularly, changes in concentrations of defense chemicals (such as monoterpenes) and nutrients (such as nitrogen and carbon) have not been compared between live trees and their cut bolts. To determine whether monoterpene and

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... ient concentrations differ after cutting, jack pine (Pinus banksiana Lamb.) trees in Lac La Biche (Alberta) were selected and sampled for phloem tissue. Then, these trees were harvested into two bolts per tree and stored at 4 °C for 3 and 6 months. Phloem was sampled from both live trees and bolts 3 and 6 months after storage. We found that major monoterpenes of jack pine were higher in phloem from bolts than from live trees. Storage time did not affect the results. Furthermore, some nutrients including nitrogen were also higher in bolts and varied between storage times. We conclude that researchers should be aware of the observed changes in the host quality that may have positive or negative effects on the development and behavior of bark beetles under observation.