Neurodegenerative diseases are associated with brain aging which leads to impaired cholinergic dysfunction. In the current study, we aimed to identify the neuroprotective effect of Clitoria ternatea (CT) methanolic leaf extract against restraint stress (RS) and aluminum (AlM) induced age-related toxicity in cerebellum of young and adult rats. In CT methanolic leaf extract 9-phytoconstituents such as 4H-1-Benzopyran-4-one, 7-hydroxy-2-(4hydroxyphenyl); Cyclopentaneundecanoic acid, methyl ester;

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... hytol; Methyl Isostearate; 12-Methyl-E, E-2,13-Octadecadien-1-ol; Cyclopropaneoctanoic acid, 2-{[2 pentylcyclopropyllmethyl}, methyl ester; Ethanol,2-[9-octadecenyloxy], [Z]; Octadecanoic acid, 5,9,13,17tetramethyl, methyl ester; Hyocholic acid were identified using Gas chromatography-mass spectrometry (GC-MS), their structures identified based on NIST data, and biological activities were specified. Further, the young (3 months age) and adult rats (12 months age) were randomized into eight groups, control, AlM, RS, CT, RS+AlM, AlM+CT, RS+CT and RS+AlM+CT administered groups. Both the age group rats were restrained in a restraint chamber for 60 min/day/30 days, AlM (100 mg/kg/30 days) and CT leaf extract (50 mg/kg/30 days) were administered orally according to their respective groups. RS and AlM administration showed significant reduced ACh and AChE Cholinergic markers levels in cerebellum compared to control group. Whereas co-administration of CT methanolic leaf extract with RS and AlM showed enhanced levels of ACh and AChE compared to RS and AlM alone treated groups. These data suggest that the rich source of phytoconstituents of CT methanolic leaf extract are responsible for anticholinergic and neuroprotective effects against RS and AlM age-related toxicity.