Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model
Journal of Functional Foods
Article: Ferreira-Lazarte, A, Montilla, A, Mulet-Cabero, A-I et al. (4 more authors) (2017) Study on the digestion of milk with prebiotic carbohydrates in a simulated gastrointestinal model. 23 24 25 37 38 39 40 41 Keywords: lactulose oligosaccharides, prebiotics, digestion, milk, galacto-42 oligosaccharides 43 65 trisaccharide 4'-galactosyl-lactose was hardly digested in vitro with a homogenate of 66 intestinal mucosa of rats. According to Torres et al. (2010), more than 90% of GOS are 67
... e to digestive enzymes and can reach the colon to exert their positive effect. 68 2.2.Obtainment of prebiotic ingredients 97 OsLu were obtained at pilot scale by Innaves S.A. (Vigo, Spain) following the 98 method described by Anadón et al. (2013). In brief, OsLu were synthesised using a 99 commercial lactulose preparation (670 g/L; Duphalac®, Abbott Biologicals B.V., Olst, 100 The Netherlands), diluted with water to 350 g/L and pH adjusted to 6.7 with KOH, and 101 -galactosidase from Aspergillus oryzae (16 U/mL; Sigma), selected by its high yield 102 for synthesis of OsLu (Cardelle-Cobas et. al., 2016). Enzymatic reactions were carried 103 out at 50 ˚C in an orbital shaker at 300 rpm for 24 h. Afterwards, samples were 104 immediately immersed in boiling water for 10 min to inactivate the enzyme. The 105 mixture of oligosaccharides (20% [w/v]) was treated with fresh Saccharomyces 106 cerevisiae (1.5% [w/v]; Levital, Paniberica de Levadura S.A., Valladolid, Spain) at 107 30ºC and aeration at 20 L/min, to decrease the monosaccharides content (Sanz et al., 108 2005). Finally, the samples were vacuum concentrated at 40 ºC in a rotary evaporator 109 (Büchi Labortechnik AG, Flawil, Switzerland). GOS syrup was kindly provided by 110 Friesland Campina Domo (Hanzeplein, The Netherlands). 111 112 2.3.Milk samples 113 Skim Milk Powder (low-heat organic, protein 42.34%, fat 0.89%, lactose 49.8% 114 (w/w) (SMP) was kindly provided by Fonterra NZ. The SMP was reconstituted at 10% 115 with distilled water and, subsequently, lactulose (Duphalac ® ), GOS or OsLu were added 116 at 5% (w/w), taking into account previous recommendations for prebiotic doses (3.3 g 117 of prebiotic carbohydrates/100 mL) (Walton et al., 2012; Whisner et al., 2013; Lopez-341 and biliary responses to solid-liquid or homogenized meals". Digestive Diseases 342 and Sciences 24: 101-110. 343 Mandalari G, Adel-Patient K, and Mills ENC. (2009). "In vitro digestibility of beta-casein and beta-347 lactoglobulin under simulated human gastric and duodenal conditions: A multi-348 laboratory evaluation". Regulatory Toxicology and Pharmacology 55: 372-381.