Caveolin-1 Expression Negatively Regulates Cell Cycle Progression by Inducing G0/G1 Arrest via a p53/p21WAF1/Cip1-dependent Mechanism

Ferruccio Galbiati, Daniela Volonte', Jun Liu, Franco Capozza, Philippe G. Frank, Liang Zhu, Richard G. Pestell, Michael P. Lisanti, Carl-Henrik Heldin
2001 Molecular Biology of the Cell  
Caveolin-1 is a principal component of caveolae membranes in vivo. Caveolin-1 mRNA and protein expression are lost or reduced during cell transformation by activated oncogenes. Interestingly, the human caveolin-1 gene is localized to a suspected tumor suppressor locus (7q31.1). However, it remains unknown whether caveolin-1 plays any role in regulating cell cycle progression. Here, we directly demonstrate that caveolin-1 expression arrests cells in the G 0 /G 1 phase of the cell cycle. We show
more » ... hat serum starvation induces up-regulation of endogenous caveolin-1 and arrests cells in the G 0 /G 1 phase of the cell cycle. Moreover, targeted down-regulation of caveolin-1 induces cells to exit the G 0 /G 1 phase. Next, we constructed a green fluorescent protein-tagged caveolin-1 (Cav-1-GFP) to examine the effect of caveolin-1 expression on cell cycle regulation. We directly demonstrate that recombinant expression of Cav-1-GFP induces arrest in the G 0 /G 1 phase of the cell cycle. To examine whether caveolin-1 expression is important for modulating cell cycle progression in vivo, we expressed wild-type caveolin-1 as a transgene in mice. Analysis of primary cultures of mouse embryonic fibroblasts from caveolin-1 transgenic mice reveals that caveolin-1 induces 1) cells to exit the S phase of the cell cycle with a concomitant increase in the G 0 /G 1 population, 2) a reduction in cellular proliferation, and 3) a reduction in the DNA replication rate. Finally, we demonstrate that caveolin-1-mediated cell cycle arrest occurs through a p53/p21-dependent pathway. Taken together, our results provide the first evidence that caveolin-1 expression plays a critical role in the modulation of cell cycle progression in vivo. † Current address:
doi:10.1091/mbc.12.8.2229 pmid:11514613 pmcid:PMC58591 fatcat:gr2sqqivljhbtl754n56rmf4sm