Diurnal Changes in the Leucogram of Ovariectomized Gilts

U. Magnusson, A.-M. Dalin
1992 Acta Veterinaria Scandinavica  
Recognition of diurnal variations in physiological systems is essential for correct interpretation of physiological and pathological changes in animals. This study shows diurnal changes in the leucogram over the day for adult pigs. The study comprised 8 healthy crossbred (Landrace x Yorkshire x Hampshire) ovariectomized gilts at an age of 10 months . The gilts had long time intravenous cath eters placed in the jugular vein and held in individual pens at the clinic. The study was performed on
more » ... 10th day after cath eter-operation. Blood was collected at 9 am, 12 noon, 3 pm, 5 pm and 8 pm in tub es containing EDTA. In these tube s, the total number of leucocytes and the number of lymphoc ytes and neutrophils number per liter blood were counted. Corti sol was determined in plasma from the heparinized blood-samples collected by the same scheme as for the leucocyte count s, but 8 days after the operation. The cortisol levels were determined by a radioimmunoassy according to Nyberg et al. (1988) . The analyses in blood were performed at the Department of Clinical Chemistry, Swedish University of Agricultural Sciences. The least square means of the leucocyte numbers obt ained from an anal ysis of variance are presented in Figure 1 . Th e total number of leucocytes was signigicantl y lower (p<0.05) at 9 am (l6.7x10 9 /litre ) than at noon
doi:10.1186/bf03547326 fatcat:4y2nhkvwpjbybhaap6qmfteoze