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This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterningdoi:10.3390/mi7050080 pmid:30404255 pmcid:PMC6189915 fatcat:tqa3k3ezlncfzc5jhkzkt64inq