Curcumin inhibits telomerase activity in human cancer cell lines

Shu-Xiang Cui, Xian-Jun Qu, Yan-Ying Xie, Ling Zhou, Munehiro Nakata, Masatoshi Makuuchi, Wei Tang
2006 International Journal of Molecular Medicine  
Curcumin, one of the major components of tumeric, the dried rhizome of Curcuma longa L, has been shown to have anti-proliferating and anti-carcinogenic properties. In this study, we examined the effects of curcumin on cell growth and telomerase activity in human cancer cell lines Bel7402, HL60 and SGC7901. Curcumin (1-32 μM) showed antiproliferating effects on these cell lines in a dose-dependent manner in vitro, and anti-tumor effects when curcumin (50-200 mg/kg) was orally administered to
more » ... mice transplanted with the cancer cells. When the cells were treated with 1 μM of curcumin for 120 h, apoptotic cells were observed by means of the adridine orange/ethidium bromide staining method, single cell microgel electrophoresis and flow cytometric analysis. On the other hand, suppression of telomerase activity in extracts of the cells treated with 1 μM of curcumin was observed by means of a telomeric repeat amplification protocol -silver staining assay. These results suggest that curcumin could suppress telomerase activity in the cancer cell lines and that the decrease of telomerase expression followed by induction of apoptosis might be involved in the anti-proliferating effect of curcumin. Cells. HL60 cells were provided by P. Yang of Experimental Hematology, Institute of Hematology, Tianjin, China. Bel7402 and SGC7901 cells were obtained from Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China. Cells were grown in RPMI-1640 supplemented with 10% heatinactivated fetal bovine serum, 2 mM glutamine, penicillin and streptomycin in a humidified atmosphere (5% CO 2 ) at 37˚C. Cells were fed every 3-4 days, and Bel7402 and
doi:10.3892/ijmm.18.2.227 fatcat:owxjoox5ebf5vgpvh7orx6imli