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Rapid Detection of Orthopoxviruses
Problems of Particularly Dangerous Infections
The aim of the study was to develop a sensitive and fast immunochemical test for the detection of orthopoxviruses (OPXV) in the "point of care" format.Materials and methods. The analyses were performed in cultured crude and purifed preparations of vaccinia virus, cowpoxvirus, rabbitpoxvirus and ectromelia virus, as well as in the blood and tissue suspensions of infected mice and rabbits. OPXV-antigen was detected by one-stage and two-stage protocols of dot-immunoassay based on flat proteindoi:10.21055/0370-1069-2021-3-106-113 fatcat:gbhtupvr7vg6pn4tzsrjup4t5i