Immunogold Techniques in Electron Microscopy [chapter]

Mogana Das Murtey
2016 Modern Electron Microscopy in Physical and Life Sciences  
Ever since electron microscopy became an important tool in the scientific research, the focus had been mainly on ultrastructural analysis with little success in the development and application of suitable techniques for the localization of macromolecules in cells. The emergence of immunogold techniques in the 1960s managed to fill this gap in serving this function. The aim of this chapter is to equip researchers, postgraduate students, and technicians with essential knowledge to utilize
more » ... ld techniques for ultrastructural investigations in the life sciences. The principles and factors involved have been highlighted to give researchers a quick review of the techniques before embarking on their ultrastructural localization procedures. The advantages and limitations of the four types of immunogold labeling techniques have been discussed.
doi:10.5772/61719 fatcat:5h5frne2hjcgvg2x2mtmrg4xde