Quality control for microbiological culture media: Is it enough to follow the NCCLS M22-A2 procedures?

Vlademir Vicente Cantarelli, Everton Inamine, Teresa Cristina Zenobin Brodt, Carina Secchi, Fabiana de Souza Pereira, Maria Cristina Amaro, Alice Aurora Batalha, Simone Dornellas Ligiero
2003 Brazilian Journal of Microbiology  
Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some
more » ... tidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised.
doi:10.1590/s1517-83822003000500003 fatcat:mx6ssrnqknbtrbalqxuynuehru