Identification and Quantification of Germacrene D from the Extracts of Zanthoxylum ovalifolium Leaves by AgNO3-TLC Densitometric Method

B.S. Theerthavathy, Shaukath Ara Khanum, S. Ravi Kiran
2019 Asian Journal of Chemistry  
Germacrene D an important sesquiterpenoid has been extracted from leaves of Zanthoxylum ovalifoilum and high performance thin-layer chromatographic method has been developed for its simultaneous identification and determination. The compound was extracted by cold extraction and separated and identified on a AgNO3 impregnated silica gel 60 F254 HPTLC plates with dichloromethane:acetone (9.8:0.2 v/v) as mobile phase. The quantification of geremcare D was carried out by densitometry scanning
more » ... med at λ = 254 nm by reflectance scanning. This facilitated a well-resolved band for the compound with a Rf value of 0.46 ± 0.01. Linear regression concentration analysis data for mean calibration plots showed good linear relationship in the range of 1000-10000 ng/mL, with a correlation coefficient, slope and intercept of 0.9985 ± 0.0004, 21.62 ± 0.435 and 3308.87 ± 10.24, respectively. The minimum amount of germacrene D that could be authentically detected (LOD) and quantified (LOQ) were determined and found to be 212.07 and 521.23 ng/spot, respectively. The method was validated by considering the parameters linearity, precision, accuracy, specificity and robustness wherein, it is evident from the results that the proposed method was simple, safe and reliable.
doi:10.14233/ajchem.2019.21871 fatcat:6ankjs76mjd7bevkd2dz4kjcwa