A naturally occurring single basic amino acid substitution in the V3 region of the human immunodeficiency virus type 1 env protein alters the cellular host range and antigenic structure of the virus

T Shioda, S Oka, S Ida, K Nokihara, H Toriyoshi, S Mori, Y Takebe, S Kimura, K Shimada, Y Nagai
1994 Journal of Virology  
Human immunodeficiency virus type 1 circulates in vivo as a mixture of heterologous populations (quasispecies). We previously analyzed the quasispecies of the third hypervariable region (V3) in the viral envelope glycoprotein gpl20 in an infected individual and found that the species with a basic amino acid substitution (lysine for aspartic acid) at a particular position evolved and became a distinct population within a short period, followed by progression to the typical immunodeficiency stage
more » ... unodeficiency stage (S. Oka et al., AIDS Res. Hum. Retroviruses 10: [271][272][273][274][275][276][277] 1994). In the present study, we examined the biological significance of this amino acid substitution by constructing recombinant viruses with specific point mutations and comparing their replication capabilities in different cell types. The results demonstrated that the single basic amino acid substitution was In a previous study, we have analyzed sequence changes of the V3 domain of HIV-1 in serum samples of a patient, which were obtained at four different times during the clinical course, which was characterized by rapid progression to AIDS and death within 8 months (26). We have also demonstrated striking temporal fluctuations of the viral quasispecies in this patient. Perhaps the most notable finding is that the sequence with a basic substitution, lysine for aspartic acid at position 323, one of the four key positions relevant to cell tropism and 7689 on May 9, 2020 by guest
doi:10.1128/jvi.68.12.7689-7696.1994 fatcat:q5okxs6lgfbrncva4vvv6hivdq