Isolation of Polyphenols from Soursop (Annona muricata L.) Leaves Using Green Chemistry Techniques and their Anticancer Effect

Valdez-Guerrero Daisy Yathzamiry, Esparza-González Sandra Cecilia, Morlett-Chávez Jesús Antonio, Nery-Flores Sendar Daniel, Flores-Gallegos Adriana Carolina, Ascacio-Valdés Juan Alberto, Rodríguez-Herrera Raúl
2021 Brazilian Archives of Biology and Technology  
Cervical cancer is classified as the fourth most common malignancy in women. Natural compounds are a therapeutic alternative in cancer therapy. The aim of the study is to isolate, fractionate, and characterize extracts obtained from soursop leaves (Annona muricata L.) and determine their cytotoxic effect against HeLa cervical cancer cells and non-carcinogenic fibroblast 3T3 cells. The phytochemicals of soursop leaves were extracted through emerging green technologies such as the novel use of
more » ... the novel use of microwave-ultrasound hybridization and the use of environmentally friendly solvents (water and ethanol), in addition to the purification of extracts enriched in polyphenols by liquid chromatography with Amberlite XAD-16. Total aqueous and ethanolic extract were purified, as well as the fraction one of each extract. The extracts recovered from soursop leaves contained kaempferol and its isomers, procyanidins, catechin, and quercetin. The viability of the cells was determined with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. HeLa and 3T3 cells were exposed to concentrations of 25, 50, 75, 100, 150, 200, and 250 ppm of a solution of soursop leaf extract powder. The MTT assay showed that soursop leaf extracts were toxic to both cell lines in general, however, the ethanolic extract at 25 and 50 ppm demonstrated inhibition in cell viability against the HeLa HIGHLIGHTS  Isolate, fractionate and characterize extracts obtained from soursop leaves.  Use of emerging green technologies such as microwave-ultrasound hybridization.  The extracts contain kaempferol, procyanidins, catechin, and quercetin.  The total ethanolic extract demonstrates cytotoxic effect on HeLa cells. 2 Valdez-Guerrero, D.Y.; et al. cancer line and low cytotoxicity for 3T3 fibroblast cells. In conclusion, the novel microwave-ultrasound hybridization technology allows the extraction of polyphenols that may have a potential cytotoxic effect on cancer cells.
doi:10.1590/1678-4324-2021200163 fatcat:vxd33giqhvbfnlyxfurzpuvhmm