A Novel Point-of-Care BioNanoSensor for Rapid HIV Detection and Treatment Monitoring

Tomasz Rozmyslowicz
2015 Journal of AIDS and Clinical Research  
We report here a new diagnostic approach to the direct detection of HIV in blood or other body fluids that is rapid, sensitive and potentially applicable in a point-of-care setting. The approach follows on the development of a novel BioNanoSensor (BNS) device that utilizes piezoelectric technology to detect the presence of the HIV surface glycoprotein gp120 in a nanoscale format. The detection range of the BNS device for the biomarker gp120 displayed a low-end sensitivity of 6.5×10 4 HIV viral
more » ... articles/ml, while using a small fluid sample (5 µl) and with a reaction time of less then 30 seconds. Performance of this device indicated that the BNS has utility for direct detection of HIV particles prior to, and independent from, antibody formation. Accordingly, this device holds utility to monitor the status of HIV infection both early after exposure to virus as well as during chronic HIV infection. The BNS parameters of small sample volume, compact device size, and detection sensitivity indicate that the BNS is potentially useful in the point-of-care and/or home setting for monitoring decisions regarding HIV treatment on a real-time basis. Recent progress in micro-electronic and micro-mechanical fabrication technologies opens exciting avenues for the development of a new class of devices to measure chemical and biological elements [17] . High frequency BioNanoSensors (BNS) are small (microchip size), solid-state devices with disk, plate or prism shapes that are implanted with a system of metal electrodes used for interfacing the sensor with electronic circuits. They are label-free, inexpensive, portable and simple to use, and can sense gases, fluids and solid materials with high accuracy and reproducibility; thus, they are well suited for applications in analytical labs as well in point-of-care settings. Among several BNS detection systems, piezoelectric Rozmyslowicz et al.
doi:10.4172/2155-6113.1000454 pmid:26457228 pmcid:PMC4596080 fatcat:tlg5fy4dabfpbdns34qc7n2rvq