PT. PG. Gorontalo is the largest sugar factory in Gorontalo province in which 10% of sugarcane waste is not utilized properly. In this study, the isolation and characterization of cellulose and α-cellulose from bagasse in the processing of alkali were 0.1M and 1.0M NaOH solution. The analysis method consisted of counterfeiting, dehemiselulose, delignification and bleaching of 10% H2O2 and 1% MgSO4. The next step was purification of cellulose to αcellulose. Cellulose content was 75%, α-cellulose

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... 37.89%. The characterization of the physicochemical properties of the product included density analysis, viscosity, 2.01% moisture content, 5.01% ash content, pH 7.7 solubility test in 1M HCl, HNO3 1M, H2SO4 0,2M, Ba(OH)2 0.5M, KOH 0.5M, CH3COOH 1M, NaOH 2M, NH4OH 1M. The IR α-cellulose spectrum showed the presence of -OH group at wave number 3419, 3331, 3344, 3360 cm-1. The C-O bond at 1635 cm-1, 1642-1649 cm-1 showed different C-O stretch vibrations in cellulose I and cellulose II fibers and C-O bonds in 1161.83 and 1063-1065 cm-1, 995-895 cm-1 showed a change in the residual l about the glycosidic bond to Cellulose II which interprets α-cellulose. Morphological analysis used SEM while the degree of crystallinity used Diffractogram XRD.