Antibody response of rainbow trout with single or double infections involving viral haemorrhagic septicaemia virus and infectious haematopoietic necrosis virus

JM Fregeneda-Grandes, HF Skall, NJ Olesen
2009 Diseases of Aquatic Organisms  
Juvenile rainbow trout Oncorhynchus mykiss were experimentally infected by immersion with viral haemorrhagic septicaemia virus (VHSV), infectious haematopoietic necrosis virus (IHNV) or with both viruses. The presence of neutralizing antibodies in the sera of infected fish were analysed by 50% plaque neutralization tests (50%PNT). In Group 1 (infected with VHSV) and Group 2 (infected with IHNV) neutralizing antibodies were found in 41% and 21% of the serum samples, respectively. No
more » ... ly. No cross-reacting antibodies were found in these 2 groups. In Group 3 (infected with both viruses) 30% of the samples showed neutralizing antibodies against VHSV, 21% against IHNV and 12% against both viruses. Fish in Group 3 developed a double specific antibody reaction whose kinetics and intensity (mean of log 10 titres) were similar to the antibody response of the single infected groups. KEY WORDS: VHSV · IHNV · Fish · Antibody response · Rainbow trout Resale or republication not permitted without written consent of the publisher Editorial responsibility: Mark Crane,
doi:10.3354/dao01993 pmid:19301633 fatcat:dczjhxbq5fet7oxmh2dfqhppr4