In the conditions of field stationary experiment on leached black soil the number of bacteria that dissolve organic forms of phosphates was studied, as well as phosphatase activity of spring barley plants rhizospheric soil under the action of fertilizers and microbial agent Mikrohumin. It was found that the studied parameters are positively affected by the introduction of fertilizers into the soil in the amounts not exceeding N60P60K60. Effective is also the first year aftereffect of 40 t/ha of

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... manure. The application of Mikrohumin enables the increase of phosphate-mobilizing bacteria and phosphatase activity of rhizospheric soil in all options of the experiment. When assessing the impact of fertilizers and biological agent interaction on crop performance the perspective of mentioned agricultural backgrounds was shown.