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Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor
Molecular Systems Biology
Metabolic heterogeneity between individual cells of a population harbors significant challenges for fundamental and applied research. Identifying metabolic heterogeneity and investigating its emergence require tools to zoom into metabolism of individual cells. While methods exist to measure metabolite levels in single cells, we lack capability to measure metabolic flux, i.e., the ultimate functional output of metabolic activity, on the single-cell level. Here, combining promoter engineering,doi:10.15252/msb.20199071 pmid:31885198 fatcat:jcud7hbqrrebnj5nfrsyijhqai