Forensic analysis: genetic identification of individuals using multiplex PCR on polymorphic STR markers. (Practical activity in the Cybertory virtual laboratory)

Angel Herráez
2020 Zenodo  
A protocol to work on the «Cybertory» virtual laboratory at Biomodel.UAH.es Genetic identity tests, often called genetic fingerprinting or DNA identification test, are based on the genetic diversity of individuals, i.e., some differ from others with respect to the nucleotide sequence present in diverse regions of the genome, a fact known as genetic polymorphism. The most frequent approach is to use polymorphic regions in the genome that do not code for any products (either protein or RNA) and,
more » ... ence, do not carry any functional advantages or disadvantages, and so their variants or alleles are distributed with high diversity in the population. This assay focuses particularly on several polymorphic regions known as short tandem repeats or STR, which belong to one of the international reference sets, the CoDIS markers, well known for being used by the FBI. The difference among alleles present in different individuals is in the number of repeats and, hence, in the length of DNA fragments resulting from PCR amplification, which will be analysed by electrophoresis. As a starting material we have (virtual) DNA samples from the crime scene (therefore, DNA belonging to the unknown criminal) and from several suspects. Aim: Using several PCR primers, directed to the polymorphic STR regions, identify the criminal by comparing the suspects with the crime scene evidence.
doi:10.5281/zenodo.3607339 fatcat:3pdbaaronrfglnmtthetblhn6y