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The phylogenetic relationships among "Barbus" species (Barbus jacksonii, B. paludinosus and B. trimaculatus) from Malagarasi and Pangani River systems were examined by partial mitochondrial cytochrome b (cyt.b) gene. Sequences of the same samples from elsewhere in Africa obtained from the South African Institute of Aquatic Biodiversity (SAIAB's) collection were also included in the analysis. Molecular technique was necessary due to phenotypic plasticity and extensive overlap of morphologicaldoi:10.4236/ns.2013.512152 fatcat:uwu26ody3fhnvchep4anrlxt4y