Commencing fibroblasts and keratinocytes cell cultures for severely burned patients is very problematic due to low viability and number of cells isolated from donor skin. These cultures last long due to the high doubling time. Number of obtained cells is often insufficient to cover the extensive burn wounds, and the waiting time for culturing slows down the healing process. The solution to this problem is permanently available source of allogeneic cells, which are easy to isolate and culture,

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... th a high number of population doubling, showing no senescence in spite of repeated passages and able to differentiate into keratinocytes and fibroblasts. Considerable source for these purposes should be human amnion. Transplants of these cells should be performed as soon as possible after defining the depth of burns in order to stimulate the healing process while waiting for end of culture of autologous fibroblasts and keratinocytes in order to improve the quality of wound healing. Moreover, due to the immunosuppressive properties amniotic cells can be used with allogeneic transplants fibroblasts and keratinocytes. The aim of this study is to verify ability of human amniotic mesenchymal stem cells (hAMMSCs) to share cluster of differentiation with fibroblast and keratinocytes in order to support autologous or allogeneic cells transplant and to verify impact of hAMMSCs on keratinocytes and fibroblasts ability to proliferation and wound healing.