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One of the major constraints in DNA computation is the exponential increase in material consumption and computation time for larger computation size in DNA computing particularly in critical stages such as initial pool generation and extraction during gel electrophoresis. In DNA computation, both the hybridization-ligation method and parallel overlap assembly method can be utilized to generate the initial pool of all possible solutions. In this paper, we discuss and compare the implementationdoi:10.7763/ijmlc.2014.v4.443 fatcat:7yqjcmdn75ek5jyy6fdx4356si