Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory

S J Read, K J Jeffery, C R Bangham
1997 Journal of Clinical Microbiology  
In this study we have devised a simple and robust PCR strategy to detect a wide range of viruses, bacteria, and parasites, all of which are capable of causing aseptic meningitis and encephalitis. The techniques developed have been used in a routine diagnostic virology laboratory to test prospectively 2,233 cerebrospinal fluid specimens. A virus was detected in 147 specimens of cerebrospinal fluid from 143 patients. Four sets of primers were sufficient to detect the virus in 135 (94%) of the
more » ... positive patients. We conclude that with appropriate primers, PCR can be systematically and economically applied to test for a range of organisms in a routine diagnostic laboratory. In our opinion, PCR will soon become the "gold standard" test for viral infections of the central nervous system.
doi:10.1128/jcm.35.3.691-696.1997 fatcat:bfoxox6wgjdkhkjz7hjpbnwfai