Bacteriological and Molecular Study of Escherichia coli Isolated from Urinary Catheters in Baquba Teaching Hospital

Ali Mahmood, Hadi Rahman Rasheed, Ammar Ahmed Sultan, Othman Jasim Mohammed
2020 Diyala Journal for Pure Science  
One Hundred fifty-two sample of catheters and urinary tracts from patients in the Urology Unit at Baquba Teaching Hospital for the period from 15/ 7/ 2018 to 30/ 9/ 2018 for different age groups ranging from 1-80 years for both sexes, were collected. Fifteen isolates from Escherichia coli were subjected to microscopic and biochemical tests and the diagnosis was confirmed by using the Vitek 2 compact system the results of the detection of some virulence factors of the selected E. coli. The
more » ... E. coli. The percentage of the ability of the bacteria to produce hemolycin enzyme was 53.33%, The ability of the isolates to form the biofilm was detected by Congo Red method and it was 40%. Resistance of the selected isolates to 7 antibiotics which belong to the Beta lactam group including Cefoxitin, Amoxicillin, Ephalexin, Ceftazidiume, Augmentin, Cefotaxime and Impinem. The ratios of antibiotic resistance were 86.7%, 73.3%, 60%, 73.3%, 40%, 40% and 66.7%, respectively. The poly chain reaction (PCR) was conducted for the detection of DNA of (8) E.coli isolates that showed resistance to the beta lactam antibiotics using primers of the genes bla SHV, bla Manuscript Code: 513D CTX-M and bla TEM. The results showed that all isolates were 100% found carriers of these genes. deportations raise your electric relay plate, so check the gel with UV exposure that you put on the ultraviolet (UV gel documentation) and photo imaging device jellies [9] . Results and discussion Investigation of some important virulence factors of E.coli Haemolysin Production All bacterial isolates were subjected to a test screening for Hemolysin enzyme. Purified colonies growing on blood agar center isolates showed hemolysin enzyme-producing regions of glycolysis transparent about developing colonies which showed eight isolates of E.coli and 53.3% of hemolysin production which matches the results of other studies [13, 14] . Biofilm formation Biofilm formation was investigated using Congo Red Agar (CRA) and dynamic Biofilm detection. The results of the current study showed that the ratio of production of biofilm was 40%. This result is not consistent with that of Al-Muaimi [15] which showed that the number of E.coli producing biological membrane with Congo red method was 23 (92%) isolating and disagreed with the findings of Abuuzaimovic et al. [16] as the ratio of production of Biofilm was 78%. Bacterial sensitivity test of antibiotics The sensitivity of the isolates under study was tested for 7 antibiotics distributed among the antibiotics which included Cefoxitin, amoxicillin, Cephalexin, Ceftazidiume, Augmentin, Cefotaxime and Impinem. E.coli cefoxitin resistance was 86.7%, which differed from the results of [17] where the ratio of insulation of resistance amounted to 26.7%. The antiamoxicillin resistance ratio was 73.3% and this result is fairly consistent with study of Al- Khalidi [14] which was 100% counter. Resistance to anti-cephalexin was 60%. These results differed from those of Abuuzaimovic et al. [16] where the rate of resistance of bacteria was Picture 1: Presence of bla TEM gene species were fractionated on 1% agarose gel electrophoresis stained with Eth.Br. Lane1:100bp DNA marker Detection of gene bla SHV The percentage of gene bla SHV appearance was (100%) Picture (2) in (8) isolates under study. This result is different from the study in [15] and the study [11], which indicates the absence of this gene in E.coli. In [21] the ratio of this gene was (5.1%). Picture 2: Presence of bla SHV gene species were fractionated on 1% agarose gel electrophoresis stained with Eth.Br. Lane1:100bp DNA marker. Detection of gene blaCTX-M The appearance of gene blaCTX-M was also (100%) Picture (3) in the isolates under study. This result is consistent with that of [20] and [22] which indicated the presence of this gene by (100%) and (88.1%) respectively, while it is not compatible with the study [21] which indicates that the ratio of gene blaCTX-M in E.coli was (7.6%). Picture 3: Presence of blaCTX-M gene species were fractionated on 1% agarose gel electrophoresis stained with Eth.Br. Lane1:100bp DNA marker.
doi:10.24237/djps.16.01.513d fatcat:b7l5yjizdneapb7gxcoalgw2gi