Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation?

Marcus da Matta Abreu, Francine Maria de Almeida, Kelli Borges dos Santos, Emílio Augusto Campos Pereira de Assis, Rafael Kenji Fonseca Hamada, Fabio Biscegli Jatene, Paulo Manuel Pêgo-Fernandes, Rogerio Pazetti
2018 Jornal Brasileiro de Pneumologia  
Objective: To evaluate whether methylene blue (MB) could minimize the effects of ischemia-reperfusion injury in the nonischemic lung on a lung transplantation rodent model. Methods: Forty female Sprague-Dawley rats were divided into 20 donors and 20 recipients. The 20 recipient rats were divided into two groups (n = 10) according to the treatment (0.9% saline vs. 1% MB solutions). All animals underwent unilateral lung transplantation. Recipients received 2 mL of saline or MB intraperitoneally
more » ... ior to transplantation. After 2 h of reperfusion, the animals were euthanized and histopathological and immunohistochemical analyses were performed in the nonischemic lung. Results: There was a significant decrease in inflammation-neutrophil count and intercellular adhesion molecule-1 (ICAM-1) expression in lung parenchyma were higher in the saline group in comparison with the MB group-and in apoptosis-caspase-3 expression was higher in the saline group and Bcl-2 expression was higher in MB group. Conclusions: MB is an effective drug for the protection of nonischemic lungs against inflammation and apoptosis following unilateral lung transplantation in rats.
doi:10.1590/s1806-37562017000000172 pmid:30517338 fatcat:wgoy5pfgrvdmddrxvk5lag76ge