STUDY OF LIPOGENESIS AND INFLAMMATION PROCESSES IN NON-ALCOHOLIC FATTY LIVER DISEASE IN THE MODEL OF STEATOSIS WITH THE USE OF HEPG2 CELLS
ИССЛЕДОВАНИЕ ПРОЦЕССОВ ЛИПОГЕНЕЗА И ВОСПАЛЕНИЯ ПРИ НЕАЛКОГОЛЬНОЙ ЖИРОВОЙ БОЛЕЗНИ ПЕЧЕНИ НА МОДЕЛИ СТЕАТОЗА С ИСПОЛЬЗОВАНИЕМ КЛЕТОК HEPG2

Elena S. Shcherbakova, State Research Institute of Highly Pure, Tatiana S. Sall, Alexander M. Ishchenko, Timur Ya. Vakhitov, Elena V. Demyanova, St Petersburg State Institute of Technology (Technical University), State Research Institute of Highly Pure, State Research Institute of Highly Pure, State Research Institute of Highly Pure, State Research Institute of Highly Pure
2019 Bulletin of the Saint Petersburg State Institute of Technology (Technical University)  
Non-alcoholic fatty liver disease (NAFLD) includes a spectrum of pathological changes in the liver, represented by steatosis (excessive accumulation of triglycerides -TG), steatohepatitis (the development of inflammation), fibrosis and cirrhosis. The article describes the making of a model of liver steatosis using HepG2 cells. The purpose of this work is to evaluate the TG and lipid accumulation and the proinflammatory cytokines production in experiments with this model. In order to make the
more » ... el of steatosis, HepG2 cells were cultured in the presence of palmitic and oleic acids; the TG content was determined with the Randox Triglycerides kit, the lipid content -with flow cytometry using Nile Red dye, the cytokine content -with ELISA. Reliable increase of the TG, lipid and IL-8 levels was observed in the model of steatosis, which is typical for this disease, compared to control. Thus, the model of steatosis obtained with HepG2 cells is an adequate in vitro model of NAFLD.
doi:10.36807/1998-9849-2019-50-76-92-96 fatcat:dpizmq2phfhwvbilzbbz4lslpy