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In this study we used ISSR molecular markers to evaluate the genetic variability of 96 tomato accessions from Banco de Germoplasma de Hortaliças (BGH) of Universidade Federal de Viçosa, Minas Gerais, Brazil. Ten ISSR primers were individually amplified to allow the differentiation of the material. All ten primers generated 144 DNA bands, 53 being polymorphic, with an average of 14.4 per primer. The profiles generated by primer 840-(GA) 8 YT contained the largest number of polymorphic bands (13doi:10.5039/agraria.v6i2a998 fatcat:x5xqpl4n6bd4zizgt3sm3y47de