This report describes a method for and the results of an analysis of cholesterol and phospholipids in HDL 2 and HDL 3 and phospholipids in VHDL (d.>L 210) of patients with cerebral thrombosis, myocardial infarction and diabetes mellitus. After precipitation and elimination of LDL (low density lipoprotein) and VLDL (very low density lipoprotein) by Na phosphotangstate (NaPhT) and MgC1 2 and bringing the non-protein density to 1. 125, serum was subjected to ultracentrifugation with #494 angle

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... r of IEC/B-60 ultracentrifuge (Kubota-International) at 105, OOOxG for 22 hours. The inadequate separation between HDL 2 and HDL 8 was observed at the clear zone beneath the top of fraction after centrifugation under these analytical conditions. Therefore, in this contaminated fraction, the HDL 2 /HDL 3 ratio was calculated by the phospholipids/cholesterol ratio which had been substantially different between HDL 2 and HDL 3 • VHDL-phospholipids was calculated by subtracting HDL 3 -phospholipids from phospholipids of density higher than 1. 125. The average HDL 2 -cholesterol and phospholipids levels were 14. 8±4. 2 and 23. 9±5. 8 mg/dl respectively in eight healthy men and 18. 8±5. 0 and 28. 5±6. 8 mg/dl respectively in seven healthy women. The average HDL 3 -cholesterol and phospholipid levels were 37. 6±7. 8 and 57. 3±11. 0 mg/dl respectively in eight healthy men and 40. 3±11. 9 and 64. 9±22.1 mg/dl respectively in seven healthy women. The average VHDL-phospholipid level was 20. 4±5. 7 mg/dl in eight healthy men and 21. 4±4. 8 mg/dl in seven healthy women. In patients with cerebral thrombosis, not only lipids in HDL 2 and HDL 3 but also VHDL-phospholipids significatly decreased. In patients with myocardial infarction, although lipids in HDL 2 and HDL 3 significantly decreased, VHDL-phospholipids maintained the normal value in most cases. In diabetic patients, HDL 2 decreased moderately but HDL 3 as largely as observed in patients with cerebral thrombosis and myocardial infarction, whereas VHDL-phospholipids were within the normal range. Among four phospholipids in VHDL fraction, lecithin and lysolecithin were significantly lower in patients with cerebral thrombosis as compared with healthy subjects.