Toxicité comparée de différentes céréales pour les sujets intolérants au gluten

L. CHARBONNIER, J. JOS, J. F. MOUGENOT, J. MOSSÉ, Claude DEMARTEAU, M. SALLANTIN, J. C. HUET
1980 Reproduction nutrition development (Print)  
Comparative toxicity of different cereals for gluten-intolerant individuals. Coeliac disease is caused by prolamines, the storage proteins of some cereals, located in the endosperm. Cereals do not all have the same toxicity. The four wheat prolamine groups (v., 3, y and M gliadins), visible in electrophoresis at acid pH, have been isolated and their toxicities compared by observing the. morphological changes in intestinal biopsies cultured in vitro when peptic-tryptic digests of the studied
more » ... of the studied proteins were added to the culture medium. The toxicity was found to be mainly located in the a and !3-gliadins and in peptides of 5 to 10 000 molecular weight. Peptides, resulting from peptic-tryptic hydrolysis, varied in length as a direct function of their proline content. In fact, peptide bond splitting by pepsin and trypsin is known to be blocked by proline. Thus, proline content determines peptide length and toxicity. Wheat, rye and barley toxicities were compared on the basis of a correlation between toxicity and the a-and P-gliadin-like prolamine contents of these cereals. Electrophoretic estimation of a-and p-gliadin-like prolamine content gave the following prediction of relative toxicity (in decreasing order) : wheat, triticale, rye, barley and oats.
doi:10.1051/rnd:19800728 fatcat:yevghpbq35gchfssxc4g4pt7nq