The misconception is the condition when someone who have an incorrect concept in understanding. If it biology prospective teachers has an incorrect concept, the student will get one, too. So, they have to develop the instrument for microteaching subject in order to diagnose the misconception. Therefore, this research aims to develop those instruments. There are two kinds of instruments were developed in this study. The first instrument evaluated a teacher performance in the microteaching

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... . The second instrument evaluated the learning devices of the teachers (eg: learning plan, resource, media, etc). There are three validators contributed in this study to validate the instruments. The result showed that the instrument was valid to be used, which has 85,71 % score for performance and 94,29% for learning devices. In the future, we can develop another kind of instrument for misconception diagnosis. Furthermore, those instruments can be applied to another subject. Kata kunci: konsep biologi, miskonsepsi, pembelajaran