The effects of folic acid on cellular senescence

Abhishek Charkraborty
2014 Journal of Student Science and Technology  
Biological functions of folic acid (FA) have been reported in various cells to include synthesis and repair of DNA. We have shown that over-dosage of FA causes blood haemolysis, leading to progressive anaemia. It is reported that 200 -400 µg/ml of FA increases the size of blood cells within 2h of treatment leading to abnormal cell division and necrosis. This observation suggests early cellular aging or senescence. We hypothesized that FA may play a vital role in cellular senescence. Kidney
more » ... blast (cos7) cells were treated with 200 µg of FA in combination with an anti-neoplastic agent, nocodazole prior to FA treatment. FA affected the expression of fibronectin (protein marker for aging) after nocodazole exposure. Furthermore, fibronectin expression was higher in FAtreated cells of the 14th generation compared to the 8th generation. 14th generation cells also showed a decrease in cell size when exposed to FA treatment. Contrasting previous results, treating cancer cells with FA showed in fact cell-beneficial effects as FA over-dosage has a noticeable effect on cell aging. However, these effects may be cell type or tissue-specific.
doi:10.13034/cysj-2014-024 fatcat:yeg4p464srhpvo3gpfcc3oxbwi