Objective: Energy metabolism is altered in the diabetic heart. However, direct in vivo evidence that diabetes impairs energetics at the chamber level is lacking. Therefore, we investigated the effect of diabetes on left ventricular (LV) energetics in a chronic ovine model. Methods: Diabetes was induced in Merino-cross sheep with streptozotocin. Experiments were performed in five animals following 12 months untreated diabetes and six animals served as controls. Open-chest anesthetized sheep were

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... instrumented to determine the LV pressure-volume relationship, oxygen consumption and free fatty acid uptake. Results: Diabetes impaired LV contractility (1.560.5 vs. 2.360.5 mmHg / ml, P,0.01). Stroke work was preserved but stroke work efficiency (stroke work / pressure-volume area) deteriorated (5264 vs. 5863%, P,0.01). Plasma free fatty acid levels increased (188561078 vs. 3546203 mmol / l, P,0.01) as did LV free fatty acid uptake (3126278 vs. 90647 mmol / beat per 100 g LV, P50.04). Contractile efficiency decreased (31.961.4 vs. 50.068.7%, P,0.01) while unloaded oxygen consumption did not change significantly. Therefore, LV oxygen utilization efficiency (stroke work / LV oxygen consumption) was compromised in the diabetic heart (14.962.8 vs. 24.364.0%, P,0.001). Conclusion: This is the first study to demonstrate that diabetes alters ventricular energetics in vivo. LV oxygen utilization efficiency is impaired as a consequence of decreased contractile efficiency and stroke work efficiency. Impaired efficiency of oxygen utilization may explain in part the increased sensitivity of the diabetic heart to ischemia and the accelerated deterioration of ventricular function in diabetic patients. 