Physalis Alkekengi Reduces Spermatogenesis and Camp-Responsive Element Modulator Gene Expression In Rat

Nasim Javdan, Jasem Estakhr
Physalis alkekengi has a large history of herbal use because of its pharmacological characteristics.The objectives of this study are to investigate effects of Physalis alkekengi extract on the structure of testis, its sperm parametic data, hormone levels(progesterone) and expression of cAMP-responsive element modulator(CREM) of adult rats. Physalis alkekengi extract was administered to adult male wistar rats (n=8) for 56 consecutive days (150 mg/kg/body weight/day). At the end of the treatment
more » ... d of the treatment period, Sperm analysis, histological and hormonal studies, and CREM gene expression were studied. Results demonstrate disorganized germinal epithelium, degenerated seminiferous tubules, decrease in the number of sperms and their motilities, increase in the number of abnormal sperms, significant decrease in the level of testosterone and nonsignificant increase in the level of concentration of plasma LH, in experimental group. The level of FSH was normal between two groups. Reverse transcription polymerase chain reaction (RT-PCR) showed a decrease in the expression of CREM in experimental group. These results suggest that Physalis alkekengi has an antispermatogenic and antifertility effects in adult male rats.