Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (WT1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry

Sana Khan, Marie Olszewski, Wei Huang, Morris Kletzel
2014 Advances in Bioscience and Biotechnology  
This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71 + CD34 + using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71 + CD34 + in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene
more » ... xpression and cellular proliferation and that GM-CSF in vitro does not create a significant difference in JMML samples.
doi:10.4236/abb.2014.52019 fatcat:kdzqx2sx3nhpzpfb627t4t4c4i