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Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (WT1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry
2014
Advances in Bioscience and Biotechnology
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This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71 + CD34 + using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71 + CD34 + in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene
doi:10.4236/abb.2014.52019
fatcat:kdzqx2sx3nhpzpfb627t4t4c4i