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DNA-Dependent Protein Kinase Phosphorylation of IκBα and IκBβ Regulates NF-κB DNA Binding Properties

Li Liu, Youn-Tae Kwak, Françoise Bex, León F. García-Martínez, Xiao-Hua Li, Katheryn Meek, William S. Lane, Richard B. Gaynor
1998 Molecular and Cellular Biology  
These results suggest that DNA-PK phosphorylation of IκBα increases its interaction with NF-κB to reduce NF-κB DNA binding properties.  ...  Furthermore, IκBα that was phosphorylated by DNA-PK was a more potent inhibitor of NF-κB binding than nonphosphorylated IκBα.  ...  We thank Dean Ballard for providing the IB␣ and IB␤ cDNAs and Cordula Kirchgessner for providing SCID and DNA-PKcs-complemented SCID cell lines.  ... 
doi:10.1128/mcb.18.7.4221 pmid:9632806 pmcid:PMC109006 fatcat:lup3k6thojdsfmqtrdnyveo65a

Specification of DNA Binding Activity of NF- B Proteins

F. Wan, M. J. Lenardo
2009 Cold Spring Harbor Perspectives in Biology  
The fine-tuning of the NF-kB DNA binding activity is essential for its fundamental function as a transcription factor.  ...  Here, we review the mechanism of the specification of DNA binding activity of NF-kB complexes, one of the most important aspects of NF-kB transcriptional control.  ...  Besides their hallmark sequestration function in cytoplasm, IkBs can also regulate the DNA binding properties of NF-kB proteins in the nucleus.  ... 
doi:10.1101/cshperspect.a000067 pmid:20066093 pmcid:PMC2773628 fatcat:aindtitmfvf6rdap6ic5mhe6eq

Antagonism of E2F-1 regulated Bnip3 transcription by NF- B is essential for basal cell survival

J. Shaw, N. Yurkova, T. Zhang, H. Gang, F. Aguilar, D. Weidman, C. Scramstad, H. Weisman, L. A. Kirshenbaum
2008 Proceedings of the National Academy of Sciences of the United States of America  
Conversely, in the absence of NF-B, or during hypoxia when NF-B abundance is reduced, basal Bnip3 gene transcription is activated by the unrestricted binding of E2F-1 to the Bnip3 promoter.  ...  We show under basal nonapoptotic conditions that NF-B constitutively occupies and transcriptionally silences Bnip3 gene transcription by competing with E2F-1 for Bnip3 promoter binding.  ...  Interestingly, however, E2F-1-mediated Bnip3 transcription was unaffected by a mutation of the p65 NF-B defective for DNA binding, indicating that DNA binding properties of p65NF-B are required to suppress  ... 
doi:10.1073/pnas.0807735105 pmid:19088195 pmcid:PMC2603431 fatcat:4a3etnzfvbh3fip2wevmqo7dda

Inhibition of NF-κB Activity by Thalidomide through Suppression of IκB Kinase Activity

Jayne A. Keifer, Denis C. Guttridge, Brian P. Ashburner, Albert S. Baldwin
2001 Journal of Biological Chemistry  
Consistent with the observed inhibition of NF-B, thalidomide blocked the cytokine-induced expression of NF-B-regulated genes such as those encoding interleukin-8, TRAF1, and c-IAP2.  ...  The transcription factor NF-B has been shown to be a key regulator of inflammatory genes such as tumor necrosis factor-␣ and interleukin-8.  ...  Thus, thalidomide may inhibit the expression of genes that are regulated by both Sp1 and NF-B. It is certain that thalidomide can inhibit the ability of NF-B to bind to the DNA.  ... 
doi:10.1074/jbc.m100938200 pmid:11297551 fatcat:d7w47ekwevhzdlmiaiky5k5vsi

Page 9 of Journal of Molecular Recognition: JMR Vol. 6, Issue 1 [page]

1993 Journal of Molecular Recognition: JMR  
Several phosphate backbone modified analogs have been synthesized and studied for their gene regulation property.  ...  The NF-«xB family of proteins include p50, p65, Rel, RelB, p105, p100, the morphogen dorsal, and share extensive homology in their DNA binding and dimerization domains.  ... 

Little Things that Count in Transcriptional Regulation

Gioacchino Natoli
2004 Cell  
Side chains Although the NF-B transcription factor tolerates sigof critical amino acids in DNA binding domains of trannificant sequence variations in its DNA binding site, scription factors make highly  ...  NF-B binding site ger, 2001).  ...  Side chains Although the NF-B transcription factor tolerates sigof critical amino acids in DNA binding domains of trannificant sequence variations in its DNA binding site, scription factors make highly  ... 
doi:10.1016/j.cell.2004.08.003 pmid:15315753 fatcat:fl2oxif6tfcmvfbtopzptazotq

Cross-species Analysis Reveals Evolving and Conserved Features of the Nuclear Factor κB (NF-κB) Proteins

Grigory Ryzhakov, Ana Teixeira, David Saliba, Katrina Blazek, Tatsushi Muta, Jiannis Ragoussis, Irina A. Udalova
2013 Journal of Biological Chemistry  
NF-B regulates transcription via binding to DNA and interactions with cofactors. Results: NF-B binding to DNA and cytosolic IBs is conserved, whereas binding to nuclear IBs has evolved.  ...  Conclusion: There is distinct evolutionary pressure on two NF-B/IB binding interfaces. Significance: The results provide inroads into IB-specific modulation of NF-B activity.  ...  With the overall conservation of NF-B/DNA binding across the animal kingdom, it is logical to speculate that Nematostella NF-B may regulate immune genes in its own host also.  ... 
doi:10.1074/jbc.m113.451153 pmid:23508954 pmcid:PMC3630861 fatcat:a5jmfg36ijhkzgchrim5urhfdm

Transcriptional Regulation by Extracellular signals: Mechanisms and Specificity

Caroline S Hill, Richard Treisman
1995 Cell  
To set the stage, we briefly discuss the different properties of transcription factors that can be modified to regulate their behavior in response to signals.  ...  These factors are introduced in Figure 1 , and their properties are summarized in Table 1 .  ...  DNA-binding properties can also be regulated by phosphorylation at remote sites, as in the growth factor-regulated changes in the DNA-binding properties of serum response factor (SRF) (Rivera et al.,  ... 
doi:10.1016/0092-8674(95)90403-4 pmid:7834740 fatcat:3uw3gnrpmvd6pb5rulnqs5hs4e

Intracellular redox regulation by a cystine derivative suppresses UV-induced NF-κB activation

Manabu Kitazawa, Takashi Nakano, Hiromi Chuujou, Eiji Shiojiri, Keiji Iwasaki, Kazutami Sakamoto
2002 FEBS Letters  
While NAC suppressed the I-U UB degradation but not the DNA binding, DACDM prevented the activated NF-U UB from binding DNA, without in£uencing the I-U UB degradation.  ...  While NF-U UB is activated through inhibitor (I)-U UB degradation by oxidative stress, its DNA binding is accelerated in the reduced state.  ...  Indeed, antioxidants increase the DNA binding of activated NF-UB [15] . Thus, the biphasic property of the NF-UB pathway can be regulated by oxidants as well as antioxidants.  ... 
doi:10.1016/s0014-5793(02)03152-6 pmid:12208514 fatcat:ekygukne7rg2pho5piwyzsrn34

In silico evaluation of Benzo (f) chromen-3-one as a potential inhibitor of NF-κB: A key regulatory molecule in inflammation-mediated pathogenesis of diabetes, Alzheimer's, and cancer

2018 Journal of Applied Pharmaceutical Science  
Compound binds to the DNA binding domain of NF-κB molecules with a binding affinity of −7.8 kcal/ mol.  ...  Biochemical studies on conserved DNA binding interactions studies by James et al. (2009) clearly demonstrated the protein DNA interactions of NF-κB (Fig. 3) .  ... 
doi:10.7324/japs.2018.81218 fatcat:5fw4ridfp5fyppopmpdckh6xce

Biochemical Characterization of the NF-Y Transcription Factor Complex during B Lymphocyte Development

R. Alexander Currie
1998 Journal of Biological Chemistry  
Recombination of the native NF-YA:B:C complex with the transcriptional cofactor, PC4, likewise conferred high affinity NF-Y binding to anion exchangers, and stabilized NF-Y interaction with CCAAT-box DNA  ...  anion exchange binding to NF-Y as an intact trimeric complex.  ...  for the NF-Y Q ϩ binding properties.  ... 
doi:10.1074/jbc.273.29.18220 pmid:9660784 fatcat:6cyf45xh7ncatdp2o4vhlqsrym

Histone-Fold Domain Protein NF-Y Promotes Chromatin Accessibility for Cell Type-Specific Master Transcription Factors

Andrew J. Oldfield, Pengyi Yang, Amanda E. Conway, Senthilkumar Cinghu, Johannes M. Freudenberg, Sailu Yellaboina, Raja Jothi
2014 Molecular Cell  
Mechanistically, NF-Y's distinct DNA-binding mode promotes master/pioneer TF binding at enhancers by facilitating a permissive chromatin conformation.  ...  Genome-wide studies in ESCs and neurons reveal that NF-Y regulates not only genes with housekeeping functions through cell type-invariant promoter-proximal binding, but also genes required for cell identity  ...  nucleosomal DNA view the NF-Y/DNA complex, with its nucleosome-like properties, as an attractive target.  ... 
doi:10.1016/j.molcel.2014.07.005 pmid:25132174 pmcid:PMC4157648 fatcat:qlvlcaykhza2hg5eyfjnjazpou

Induction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts

A S Baldwin, J C Azizkhan, D E Jensen, A A Beg, L R Coodly
1991 Molecular and Cellular Biology  
A DNA-binding factor with properties of NF-kappa B and another similar activity are rapidly induced when growth-arrested BALB/c 3T3 cells are stimulated with serum growth factors.  ...  These DNA-binding activities bind many of the expected NF-kappa B target sequences, including elements in the class I major histocompatibility complex and human immunodeficiency virus enhancers, as well  ...  Since these properties are characteristic of NF-KB (7, 60), we next analyzed the binding specificities of the serum growth factor-induced DNA-binding factors.  ... 
doi:10.1128/mcb.11.10.4943 pmid:1922027 pmcid:PMC361472 fatcat:qkwcfzcgpbcw3oi7pfeezrd5qm

Genomic Analysis Reveals a Novel Nuclear Factor-κB (NF-κB)-binding Site in Alu-repetitive Elements

Athina Antonaki, Constantinos Demetriades, Alexander Polyzos, Aggelos Banos, Giannis Vatsellas, Matthieu D. Lavigne, Effie Apostolou, Eva Mantouvalou, Deppie Papadopoulou, George Mosialos, Dimitris Thanos
2011 Journal of Biological Chemistry  
The transcription factor NF-B is a critical regulator of immune response pathways. Results: 11% of total NF-B binding events in humans occur at the Alu-B element present in Alu repeats.  ...  The transcription factor NF-B is a critical regulator of immune responses.  ...  NF-B DNA binding (also see below).  ... 
doi:10.1074/jbc.m111.234161 pmid:21896491 pmcid:PMC3207430 fatcat:766rkn257fab3jwqp6rkpcmgni

Regulation of c-Rel Nuclear Localization by Binding of Ca2+/Calmodulin

A. Antonsson, K. Hughes, S. Edin, T. Grundstrom
2003 Molecular and Cellular Biology  
CaM can therefore differentially regulate the activation of NF-B/Rel proteins following stimulation.  ...  The NF-B/Rel family of transcription factors participates in the control of a wide array of genes, including genes involved in embryonic development and regulation of immune, inflammation, and stress responses  ...  This DNA binding activity could be stimulated by GTP (data not shown), a previously reported property of NF-B (36). Thus, a nuclear protein(s) with NF-B-like DNA binding properties binds CaM.  ... 
doi:10.1128/mcb.23.4.1418-1427.2003 pmid:12556500 pmcid:PMC141150 fatcat:xr47jqwabzgp3nggfu4ifiwbhm
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